Comparative analyses of industrial-scale human platelet lysate preparations
نویسندگان
چکیده
منابع مشابه
Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications.
BACKGROUND AIMS. Dental pulp stromal cells (DPSC) are considered to be a promising source of stem cells in the field of regenerative therapy. However, the usage of DPSC in transplantation requires large-scale expansion to cater for the need for clinical quantity without compromising current good manufacturing practice (cGMP). Existing protocols for cell culturing make use of fetal bovine serum ...
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BACKGROUND Standard protocols are lacking for the preparation of platelet lysates (PL) as an alternative to using fetal bovine serum as a cell culture supplement. This study aimed to establish optimum conditions for preparing PL for use in cell cultures. METHODS Cell density in three pooled platelet concentrates (PC) were adjusted to 1×10(12)/L and 2×10(12)/L. PL was prepared from PC by 1 to ...
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This was Presented in 5th International Congress of Iranian Iranian Society of Knee Surgery, Arthroscopy, and Sports Traumatology (ISKAST), 14-17 Feb 2018- Kish, Iran
متن کاملAutologous Platelet-Rich Plasma Preparations
Methods: PRP was collected from patients receiving NSAIDs after elective orthopaedic surgery, and platelet function was evaluated using light transmission aggregometry (LTA). Results were compared with those obtained from healthy volunteers without a history of NSAID intake during the previous 2 weeks. Two different systems for blood collection and PRP production (Arthrex ACP double-syringe sys...
متن کاملDonor Age of Human Platelet Lysate Affects Proliferation and Differentiation of Mesenchymal Stem Cells
The regenerative potential declines upon aging. This might be due to cell-intrinsic changes in stem and progenitor cells or to influences by the microenvironment. Mesenchymal stem cells (MSC) raise high hopes in regenerative medicine. They are usually culture expanded in media with fetal calf serum (FCS) or other serum supplements such as human platelet lysate (HPL). In this study, we have anal...
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ژورنال
عنوان ژورنال: Transfusion
سال: 2017
ISSN: 0041-1132
DOI: 10.1111/trf.14324